ApoE4 increases glycogen synthase kinase 3β expression and Tau phosphorylation in U87 cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 904-908, 2016.
Article
en Zh
| WPRIM
| ID: wpr-286876
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the relations among apolipoprotein E4, Tau protein and glycogen synthase kinase 3β (GSK-3β).</p><p><b>METHODS</b>U87 cells were transfected with pIRES-EGFP (control) or the recombinant plasmids ApoE4/pIRES-EGFP or ApoE3/pIRES-EGFP, and the expression levels of p-Tau/Tau and GSK-3β in the cells were examined with Western blotting. To further confirm the effect of ApoE on GSK-3β and p-Tau expressions, a short interfering RNA (siRNA) targeting ApoE (ApoE-siRNA) was transfected into U87 cells via Lipofectamine 2000 and the protein expressions were examined 24 h later.</p><p><b>RESULTS</b>Compared with those in the control group, the expressions levels of both GSK-3β and p-Tau/Tau increased significantly in the cells transfected with ApoE4 and ApoE3 plasmids (P<0.01), and the ApoE4 plasmid produced a more potent effect than the ApoE3 plasmid on the protein expressions (P<0.01). ApoE knockdown resulted in significantly reduced expressions of GSK-3β (P<0.001) and p-Tau (P<0.01) in the cells.</p><p><b>CONCLUSION</b>ApoE4 can enhance Tau phosphorylation though upregulating GSK-3β, which sheds light on a new role of ApoE4 in Alzheimer's disease.</p>
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Asunto principal:
Fosforilación
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Transfección
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Línea Celular
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Proteínas tau
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Silenciador del Gen
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ARN Interferente Pequeño
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Apolipoproteína E3
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Apolipoproteína E4
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Enfermedad de Alzheimer
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Glucógeno Sintasa Quinasa 3 beta
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2016
Tipo del documento:
Article