The expression of calcitonin gene-related peptide on the neurons associated Zusanli (ST 36) in rats / 中国结合医学杂志
Chinese journal of integrative medicine
; (12): 630-634, 2015.
Article
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| WPRIM
| ID: wpr-287123
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ABSTRACT
<p><b>OBJECTIVE</b>To investigate the biochemical characteristic of the neurons associated Zusanli (ST 36) in the rat by using Alexa Fluor 594 conjugated cholera toxin subunit B (AF594-CTB) neural tracing and calcitonin gene-related peptide (CGRP) fluorescent immunohistochemical techniques.</p><p><b>METHODS</b>Four male Sprague Dawley rats were injected with AF594-CTB into the corresponding area of the Zusanli in the human body. After 3 surviving days, the rat's spinal cord and dorsal root ganglia (DRGs) at lumbar segments were dissected following perfusion with 4% paraformaldehyde, cut into sections, and then stained with CGRPfluorescent immunohistochemical method.</p><p><b>RESULTS</b>AF594-CTB labeled sensory neurons were detected in the L3-L6 DRGs with high concentration in L4 DRG, and the labeled motor neurons located in the dorsolateral and intermediate regions of lamina IX from L3-L5 segments with high concentration at L4. Meanwhile, CGRPpositive neural labeling distributed symmetrically on both sides of DRGs, anterior and dorsal horns of spinal cord. In the AF594-CTB labeled neurons, 37% sensory neurons and 100% motor neurons expressed CGRPpositive.</p><p><b>CONCLUSION</b>These findings present the morphological evidence to demonstrate that the sensory and motor neurons associated Zusanli in the rat distributed with segmental and regional patterns, and contained CGRP-expression.</p>
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Asunto principal:
Puntos de Acupuntura
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Péptido Relacionado con Gen de Calcitonina
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Ratas Sprague-Dawley
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Metabolismo
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Neuronas
Límite:
Animals
Idioma:
En
Revista:
Chinese journal of integrative medicine
Año:
2015
Tipo del documento:
Article