Experimental studies of human adipose tissue-derived stromal cells transfected with ad-hBMP-2 gene / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 195-198, 2006.
Article
en Zh
| WPRIM
| ID: wpr-288971
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the possibility of adipose-derived stromal cells (ADSCs) transfeced by adenovirus containing human bone morphogenetic protein-2 (Ad-hBMP-2) gene and their osteogenic potential.</p><p><b>METHODS</b>ADSCs were obtained from inguinal fat tissue of 4 weeks old SD rats. After exposure to adenovirus containing green fluorescent protein(Ad-GFP), fluorescent microscope was used to observe gene transfection effect once 12 hours. After transfected with Ad-hBMP-2, cytochemistry, immmucytochemistry and Western blot were used to examine the expression of alkaline phosphatase (ALP), osteocalcin (OC) and hBMP-2.</p><p><b>RESULTS</b>After exposed to Ad-GFP 12 hours, 52% ADSCs were observed being transfected and 48 hours later reached 95%. The double number time belonged after transfecting with Ad-hBMP-2, and cytochemistry, immucytochemistry and Western blot examines indicated positive results of ALP, OC, hBMP-2 after 48 hours.</p><p><b>CONCLUSION</b>Adipose tissue contains abundant ADSCs which could be transfected as gene vectors by adenovirus, ADSCs transfected with Ad-hBMP-2 can convert to ostoeblasts, and can act as a kind of seed cells for osteo-tissue engineering.</p>
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Índice:
WPRIM
Asunto principal:
Transfección
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Células Cultivadas
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Adenoviridae
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Tejido Adiposo
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Células del Estroma
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Ratas Sprague-Dawley
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Adipocitos
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Ingeniería de Tejidos
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Proteína Morfogenética Ósea 2
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Vectores Genéticos
Límite:
Animals
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Humans
Idioma:
Zh
Revista:
West China Journal of Stomatology
Año:
2006
Tipo del documento:
Article