Construction of Nude Mouse Model of Triple Negative Breast Cancer and Value of Thermal Tomography / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 198-204, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-289881
ABSTRACT
<p><b>OBJECTIVE</b>To explore the early detection of breast cancer by ultrasonic imaging and thermal tomography of luciferase or green fluorescent protein (GFP)-labeled MDA-MB-231 breast cancer cell line-xenografts in nude mice.</p><p><b>METHODS</b>Fluorescence-tagged lentiviral vectors were transfected into the triple-negative breast cancer cell line MDA-MB-231. These cells were implanted either subcutaneously under the right breast pad or intravenously into the tail vein of nude BALB/C mice. Thermal tomography and ultrasound imaging were used to detect tumor formation and to monitor tumor growth and metastasis in vivo.</p><p><b>RESULTS</b>Triple negative breast cancer cell line-xenografts were used to successfully construct an orthotopic nude mice model of breast cancer metastasis in the peritoneum. Thermal tomography and ultrasound imaging were used together to detect small tumors. Thermal tomography imaging detected small tumors earlier than ultrasound imaging.</p><p><b>CONCLUSIONS</b>Thermal tomography can be used to monitor changes in tumor growth and detect abnormal tissue. Therefore, it can serve as a convenient,rapid,sensitive, and reliable technique for early screening of human breast cancer.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Diagnóstico por Imagen
/
Tomografía Computarizada por Rayos X
/
Ultrasonografía
/
Línea Celular Tumoral
/
Diagnóstico
/
Modelos Animales de Enfermedad
/
Neoplasias de la Mama Triple Negativas
/
Ratones Endogámicos BALB C
/
Ratones Desnudos
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2016
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS