Ovine Follistatin gene expression and functional analysis of follistatin domains / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1050-1056, 2010.
Article
en Zh
| WPRIM
| ID: wpr-292172
Biblioteca responsable:
WPRO
ABSTRACT
In order to study ovine follistatin function, we amplified the total of 1038 base pair of ovine complete follistatin cDNA and cloned into pGEM-T vector by RT-PCR from ovine ovary RNA. After removal of the signal peptide it was subcloned into the pET41a to construct the prokaryotic expression vector, named pFSsig-. SDS-PAGE and Western blotting identified the 66 kDa product of the expression of follistatin cDNA. Based on the complete CDS sequence, we cloned follistatin N-terminal domain and domain 1 with PCR and inserted into pLEX-MCS lentiviral vector, named pFS-N+D1. After package and passage of lentivirus in 293T cells, and then infected sheep primary muscle cells (SPMC). The expression of FS N+D1 in SPMC was assayed by Western blotting. The cell growth curve of the infected SPMC and noninfected control cells displayed that FS N+D1 stablly transfected SPMC proliferated significantly faster than the control cells (P < 0.01). Our data inferred that ovine FS N+D1 domain had the function to stimulate sheep muscle cell growth.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Ovario
/
Células Procariotas
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Proteínas Recombinantes
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Ovinos
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Células Cultivadas
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Estructura Terciaria de Proteína
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Lentivirus
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Músculo Esquelético
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Biología Celular
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Folistatina
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2010
Tipo del documento:
Article