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Analysis of KIR3DL1 expression levels on 92 cases of normal donors for hematopoietic stem cells transplant / 中华血液学杂志
Chinese Journal of Hematology ; (12): 994-998, 2015.
Article en Zh | WPRIM | ID: wpr-296101
Biblioteca responsable: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study KIR3DL1 expression level on NK cell surface of normal donors for hematopoietic stem cell transplantation(HSCT).</p><p><b>METHODS</b>Ninety- two donors were performed by using of KIR genotyping, HLA high resolution genotyping and KIR3DL1 expression level using sequencebased testing(SBT), PCR- sequence specific primer(SSP)and flow cytometry methods.</p><p><b>RESULTS</b>In 92 donors, the frequencies of KIR-A/A, Bx1, Bx2 for common genotypes were 46.74%(43/92), 18.48% (17/92)and 9.78%(9/92)respectively(P<0.001); KIR-A, B1, B2, B3 for common KIR haplo-type were 70.33%(128/182), 10.99%(20/182), 7.14%(13/182) and 4.39%(8/182) respectively(P<0.001); the frequencies of HLA-BW4/BW4, HLA-BW4/BW6, BW6/BW6 ligands were 13.79%, 67.81% and 18.39% respectively(P<0.001). KIR3DL1 middle expression level among haplo- type KIR- A/A and KIR- Bx, KIR-B/B were 18.77%(3.11%-49.24%), 13.14%(1.70%-63.32%)and 0.37%(0.20%-2.60%)respectively (P<0.05). KIR3DL1 expression level[18.77%(3.11%-49.24%)]in haplo-type KIR-A/A was higher than haplo-type KIR-Bx at the same time did not express 2DL2 group[11.20%(3.50%-36.08%)](P=0.019). KIR3DL1 expression level in recognition group(HLA-BW4 positive group)[17.61%(1.40%-49.24%)] was higher than KIR3DL1 unrecognized group(HLA-BW4 negative group)[10.60%(3.50%-18.56%)] (P=0.006).</p><p><b>CONCLUSION</b>The expression levels of KIR3DL1 in different KIR genotypes, haplotypes and HLA ligands were statistically significance.</p>
Asunto(s)
Texto completo: 1 Índice: WPRIM Asunto principal: Donantes de Tejidos / Haplotipos / Células Madre Hematopoyéticas / Células Asesinas Naturales / Antígenos HLA-B / Trasplante de Células Madre Hematopoyéticas / Receptores KIR3DL1 / Genética / Genotipo / Ligandos Límite: Humans Idioma: Zh Revista: Chinese Journal of Hematology Año: 2015 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Asunto principal: Donantes de Tejidos / Haplotipos / Células Madre Hematopoyéticas / Células Asesinas Naturales / Antígenos HLA-B / Trasplante de Células Madre Hematopoyéticas / Receptores KIR3DL1 / Genética / Genotipo / Ligandos Límite: Humans Idioma: Zh Revista: Chinese Journal of Hematology Año: 2015 Tipo del documento: Article