Reversal of multidrug resistance in HL-60, MSC and CD34(+) cells from umbilical cord blood by sustained intracellular acidification / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 666-670, 2011.
Article
en Zh
| WPRIM
| ID: wpr-313920
Biblioteca responsable:
WPRO
ABSTRACT
The aim of this study was to investigate the effect of intracellular acidification on accumulation of rhodamine 123 (rh123) in non-mature cells with none or low expression of multidrug resistance MDR1. The expression of MDR1 mRNA was detected by real-time quantitative RT-PCR. Confocal laser microscopy was used to determine the calibration curve of intracellular acidification (pHi). MTT assay was used to detect the cytotoxicity of intracellular acidification on HL-60, MSC and CD34(+) cells from umbilical cord blood. Flow cytometry was applied to measure the influence of intracellular acidification. The results indicated that the intracellular acidification had no obvious cytotoxicity on HL-60, MSC and CD34(+) cells. The acidification resulted in the increased rhodamine 123 accumulation in HL-60, MSC and CD34(+) cells without P-gp activity. Moreover, the more primitive cells, the less accumulation of intracellular Rh123 were observed. It is concluded that the intracellular acidification can reverse the MDR of HL-60, MSC and CD34(+) cells.
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Índice:
WPRIM
Asunto principal:
Células de la Médula Ósea
/
Fenómenos Fisiológicos Celulares
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Miembro 1 de la Subfamilia B de Casetes de Unión a ATP
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Resistencia a Múltiples Medicamentos
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Células HL-60
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Resistencia a Antineoplásicos
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Antígenos CD34
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Biología Celular
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Sangre Fetal
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Células Madre Mesenquimatosas
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2011
Tipo del documento:
Article