Detection of human parvovirus B19, human bocavirus and human parvovirus 4 infections in blood samples among 95 patients with liver disease in Nanjing by nested PCR / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 135-137, 2013.
Article
en Zh
| WPRIM
| ID: wpr-318082
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the infection of human parvovirus B19, human bocavirus (HBoV) and human parvovirus 4 (PARV4) in blood samples among patients with liver disease in Nanjing by molecular detection.</p><p><b>METHODS</b>Nested PCR assays were designed and validated to detect B19, HBoV and PARV4, respectively. The assays were used to screen three parvoviruses in blood samples from 95 patients with different liver disease in Nanjing. The parvovirus infection was analyzed statistically.</p><p><b>RESULTS</b>The detection limits were 10 copies of genomic DNA equivalents per reaction for each assays and the good specificity were observed. The frequency of B19 and HBoV were 2/95 (2.1%) and 9/95 (9.5%) in blood samples respectively. No PARV4 was detected. HBoV was detected in 3/5 patients with drug-induced hepatitis.</p><p><b>CONCLUSION</b>Both B19 and HBoV infection were detected in blood from patients with liver disease.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Viremia
/
Virología
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Reacción en Cadena de la Polimerasa
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Parvovirus B19 Humano
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Parvovirus
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Bocavirus Humano
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Coinfección
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Hepatopatías
Tipo de estudio:
Diagnostic_studies
Límite:
Adolescent
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Adult
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Aged
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Child
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2013
Tipo del documento:
Article