Chemical coupling of anti-dNA antibody on collagen coating / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 718-722, 2005.
Article
en Zh
| WPRIM
| ID: wpr-318829
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the feasibility and stability of chemically conjugating IgM on collagen films.</p><p><b>METHODS</b>IgM was labeled with 125I using the chloramine-T method. Six collagen films were randomly divided into two groups. In chemical coupling group 125I-labeled IgM was chemically coupled with the films through N-succinmiclyl-3- (2-pyridyl-dithio) propionate reaction. In control group 125I-labeled IgM was absorbed onto collagen films. The amount of IgM on the collagen films and the amount of IgM remained on the films after extensive rinsing with phosphate buffered saline were monitored by counting the radioactivity of 125I.</p><p><b>RESULTS</b>The amount of antibodies loaded onto collagen films in the chemical coupling group was 15 times higher than that on the control films, showing significant statistical difference (P < 0.01). And the stability of conjugation antibodies on collagen films was significantly better than the control films.</p><p><b>CONCLUSION</b>Chemical coupling is an effective approach to immobilize antibodies on collagen for further plasmid DNA tethering.</p>
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WPRIM
Asunto principal:
Unión Proteica
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Propiedades de Superficie
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Técnicas In Vitro
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Inmunoglobulina M
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Angioplastia Coronaria con Balón
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Stents
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Química
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Anticuerpos Antinucleares
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Colágeno
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Materiales Biocompatibles Revestidos
Límite:
Animals
Idioma:
Zh
Revista:
Acta Academiae Medicinae Sinicae
Año:
2005
Tipo del documento:
Article