Activation of PACAP receptor could protect cultured hippocampal neurons against beta amyloid peptide induced neurotoxicity / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 145-148, 2002.
Article
en Zh
| WPRIM
| ID: wpr-319354
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>AIM</b>To observe the protective role of pituitary adenylate cyclase activating polypeptide (PACAP) on hippocampal neuronal apoptosis induced by beta amyloid peptide in the culture.</p><p><b>METHODS</b>Hippocampal neurons were isolated from 1d old SD rat and neuronal survival and apoptosis were measured by MTT assay and DNA ladder.</p><p><b>RESULTS</b>25 micromol/L Abeta could induce neuron apoptosis while co-treatment with PACAP could increase the survival of hippocampal neurons. The antagonist of PACAP receptor, P6-27, could reverse the effect of PACAP.</p><p><b>CONCLUSION</b>PACAP could protects cultured neurons from the neurotoxicity of Abeta through the activation of PACAP receptor and may have a bright use in treatment of neurodegenerative disease.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Células Cultivadas
/
Péptidos beta-Amiloides
/
Ratas Sprague-Dawley
/
Apoptosis
/
Biología Celular
/
Receptores del Polipéptido Activador de la Adenilato-Ciclasa Hipofisaria
/
Toxicidad
/
Hipocampo
/
Metabolismo
/
Neuronas
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Año:
2002
Tipo del documento:
Article