Cloning, expression and purification of SARS coronavirus PUMC2 strain nucleocapsid protein / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 504-507, 2003.
Article
en Zh
| WPRIM
| ID: wpr-327050
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To clone, express and purify nucleocapsid protein from SARS coronavirus PUMC2 strain.</p><p><b>METHODS</b>According to the published SARS coronavirus genome sequences, the full length cDNA of N protein from SARS coronavirus PUMC2 strain was cloned by RT-PCR and the cDNA was cloned into the pET32a expression vector. The recombinant N protein was expressed in E. coli BL21 (DE3), and purified by Ni(2+)-NTA.</p><p><b>RESULTS</b>Prokaryoticly expressed and purified N protein of SARS coronavirus PUMC2 strain was obtained.</p><p><b>CONCLUSIONS</b>The SARS coronavirus recombinant N protein obtained by genetic engineering methods can be used for further functional study of SARS coronavirus N protein.</p>
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Índice:
WPRIM
Asunto principal:
Proteínas Recombinantes de Fusión
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ADN Viral
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ARN Viral
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Datos de Secuencia Molecular
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Secuencia de Bases
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Secuencia de Aminoácidos
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Genoma Viral
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Clonación Molecular
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Análisis de Secuencia de ADN
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ADN Complementario
Idioma:
Zh
Revista:
Acta Academiae Medicinae Sinicae
Año:
2003
Tipo del documento:
Article