Expression of origin recognition complex 1 gene in DNA replication of vascularsmooth muscle cells / 中华病理学杂志
Chinese Journal of Pathology
; (12): 122-125, 2007.
Article
en Zh
| WPRIM
| ID: wpr-333955
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of origin recognition complex 1 (ORC1) during the DNA replication of vascular muscle cells (VSMC).</p><p><b>METHODS</b>VSMC of thoracic aorta in rats were obtained by the adherence method of tissue culture. The cell synchrony was obtained by the method of double-thymidine block, colchicine treatment and serum starvation. The expression of ORC1 mRNA at different cell cycles of VSMC was determined by RT-PCR and the protein expression of ORC1 was analyzed by Western blot.</p><p><b>RESULTS</b>Cultured VSMC were identified by light microscope and immunocytochemistry. Significant expression of ORC1 mRNA and protein in a quiescent stage of VSMC were not observed. Upon synchronization, the expression of ORC1 mRNA was significantly higher at G(1)/S phase of VSMC than that at S and G(2)/M phases. The expression of ORC1 protein followed same changes as the ORC1 mRNA expression at different stages of cell cycles.</p><p><b>CONCLUSION</b>ORC1 may be an important regulatory factor at the initiation of proliferative process of VSMC.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Aorta Torácica
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Fisiología
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ADN
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ARN Mensajero
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Regulación Neoplásica de la Expresión Génica
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Ciclo Celular
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Células Cultivadas
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Western Blotting
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Ratas Sprague-Dawley
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Pathology
Año:
2007
Tipo del documento:
Article