c-SRC knockdown decreases phosphorylated STAT3 expression and viability of HeLa cells / 生理学报
Acta Physiologica Sinica
; (6): 198-204, 2011.
Article
en Zh
| WPRIM
| ID: wpr-336001
Biblioteca responsable:
WPRO
ABSTRACT
The present study was to determine the effect of c-SRC on the viability of human cervical cancer HeLa cells and the expression of phosphorylated signal transducer and activator of transcription-3 (p-STAT3) of the cell. Post-transfection of c-SRC RNA interference vector, RT-PCR and Western blot were utilized to observe the contents of c-SRC mRNA and protein, respectively, in HeLa cells. The MTT was used to observe the viability of the cells. Cell cycle was observed by flow cytometry. The content of p-STAT3 in the cells was also investigated after knockdown of c-SRC. Knockdown of c-SRC significantly decreased the contents of c-SRC mRNA and protein in the cells. The viability of the cells decreased by 23.1%, 29.3%, 38.6% and 45.0% (all P < 0.05), respectively, after the cells were transfected with c-SRC RNA interference vector for 24, 48, 72, and 96 h. The number of S-phase cells decreased by 5.6%, 10.0%, 15.2% and 19.9% (all P < 0.05), respectively, after transfection of c-SRC RNA interference vector for 24, 48, 72, and 96 h. The content of p-STAT3 also decreased when c-SRC was knockdowned. Compared with the control group, after treatment of HeLa cells with STAT3 inhibitor Piceatannol for 24, 48, 72, and 96 h, the cell viability decreased by 23.8%, 29.7%, 37.3% and 45.4% (all P < 0.05), respectively, while increase of c-SRC content could not reverse the inhibitory effect. These results suggest that the inhibited viability of HeLa cells caused by knockdown of c-SRC is associated with the decreased content of p-STAT3 protein.
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Índice:
WPRIM
Asunto principal:
Fosforilación
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ARN Mensajero
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Células HeLa
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Transfección
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Regulación Neoplásica de la Expresión Génica
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Supervivencia Celular
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Genes src
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Factor de Transcripción STAT3
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Técnicas de Silenciamiento del Gen
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Genética
Límite:
Female
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Humans
Idioma:
Zh
Revista:
Acta Physiologica Sinica
Año:
2011
Tipo del documento:
Article