Overexpression of FKS1 to improve yeast autolysis-stress / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1344-1354, 2015.
Article
en Zh
| WPRIM
| ID: wpr-337486
Biblioteca responsable:
WPRO
ABSTRACT
With the development of high gravity brewing, yeast cells are exposed to multiple brewing-associated stresses, such as increased osmotic pressure, enhanced alcohol concentration and nutritional imbalance. These will speed up yeast autolysis, which seriously influence beer flavor and quality. To increase yeast anti-autolytic ability, FKS1 overexpression strain was constructed by 18S rDNA. The concentration of β-1,3-glucan of overexpression strain was 62% higher than that of wild type strain. Meantime, FKS1 overexpression strain increased anti-stress ability at 8% ethanol, 0.4 mol/L NaCl and starvation stress. Under simulated autolysis, FKS1 showed good anti-autolytic ability by slower autolysis. These results confirms the potential of FKS1 overexpression to tackle yeast autolysis in high-gravity brewing.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Saccharomyces cerevisiae
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Autólisis
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Cerveza
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Hipergravedad
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Biología Celular
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Proteínas de Saccharomyces cerevisiae
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Equinocandinas
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Genética
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Glucosiltransferasas
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Proteínas de la Membrana
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2015
Tipo del documento:
Article