Inducible expression of hypoxia-inducible factor 1alpha on the proliferation and invasion property of HepG2 cells under normoxia in vitro / 中华外科杂志
Chinese Journal of Surgery
; (12): 1634-1636, 2007.
Article
en Zh
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| ID: wpr-338095
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ABSTRACT
<p><b>OBJECTIVE</b>To study the inducible expression of hypoxia-inducible factor 1alpha (HIF-1alpha) on the proliferation and invasion property of HepG2 cells under normoxia in vitro.</p><p><b>METHODS</b>Constructed the HepG2(Tet-on-HIF-1alpha) cell line which could induce the expression of HIF-1alpha by doxycycline; Under normoxia in vitro, MTT assay was used to observe the proliferative and adhesive activity of cells, and the invasive activity was determined by transwell cell culture chamber method.</p><p><b>RESULTS</b>Under normoxia, the HIF-1alpha mRNA and protein of HepG2(Tet-on-HIF-1alpha) cells could be induced up to (5.899 +/- 2.176) and (2.179 +/- 0.742) folds by doxycycline (1 microg/ml); There were no difference of A(490 nm) between the Dox(+)and Dox(-) group in experiment detecting the proliferation activity (P > 0.05); But in adhesive experiment, the A(490 nm) of Dox (+) group was 0.662 +/- 0.058, higher than the Dox(-) group 0.526 +/- 0.808 (P = 0.008); The invasive cell number of Dox(+) group was 37.611 +/- 8.424, but in the Dox(-) group, the number was 25.333 +/- 8.117 (P < 0.01).</p><p><b>CONCLUSIONS</b>Under normoxia in vitro, the Tet-on gene regulate system could increase the HIF-1alpha protein by inducing the HIF-1alpha mRNA; HIF-1alpha has no influence with the proliferation activity, but it could enhance the adhesive and invasive properties of HepG2 cells.</p>
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Asunto principal:
Patología
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Fisiología
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ARN Mensajero
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Regulación Neoplásica de la Expresión Génica
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Adhesión Celular
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Movimiento Celular
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Supervivencia Celular
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Western Blotting
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Carcinoma Hepatocelular
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Surgery
Año:
2007
Tipo del documento:
Article