Relationship between amyloid beta-protein and oxidative stress and the protective role of pituitary adenylate cyclase activating polypeptide against oxidative stress induced damage on neuro-2a cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 171-174, 2003.
Article
en Zh
| WPRIM
| ID: wpr-339648
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>AIM</b>To observe the relationship between amyloid beta-protein (Abeta) and oxidative stress and the protective role of pituitary adenylate cyclase activating polypeptide (PACAP, PACAP-27) against damage induced by oxidative stress (H2O2) in neurem-2a cells.</p><p><b>METHODS</b>With cultured neuro-2a cells the cell survival and apoptosis were measured by MTT assay, Hoechest33258 staining, DNA ladder and the percentage of small DNA fragment.</p><p><b>RESULTS</b>Concentration-dependent toxicity was induced with H2O2 treatment for 24 h. The neurotoxicity of H2O2 was increased by about 10 times with cotreatment neurons with amyloid beta-protein fragment 25-35 (Abeta(25-35)). While decrease the percentage of small DNA fragmentation the cell survival was increased with co-treatment with PACAP-27(which were added to the culture everyday). The effect of PACAP was not reversed with antagonist of PACAP receptor, PACAP(6-27).</p><p><b>CONCLUSION</b>Abeta and H2O2 can promote each other's neurotoxicity. Cultured neurons were protected by PACAP27 from the neurotoxicity of H2O2 but not through the activation of PACAP-27 receptor.</p>
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Índice:
WPRIM
Asunto principal:
Farmacología
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Supervivencia Celular
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Células Cultivadas
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Péptidos beta-Amiloides
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Apoptosis
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Estrés Oxidativo
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Biología Celular
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Polipéptido Hipofisario Activador de la Adenilato-Ciclasa
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Toxicidad
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Peróxido de Hidrógeno
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Año:
2003
Tipo del documento:
Article