Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus field strains in central China during 2010-2012 outbreaks / 病毒学报
Chinese Journal of Virology
; (6): 197-205, 2013.
Article
en Zh
| WPRIM
| ID: wpr-339952
Biblioteca responsable:
WPRO
ABSTRACT
Since late 2010, porcine epidemic diarrhea virus (PEDV) has been re-emerging in central China. To explore the possible reason of the PEDV outbreaks, twelve PEDV field strains were isolated from different swine breeding farms in central China during 2010-2012, and molecular diversity, phylogenetic relationships of these strains with other PEDV reference strains were investigated. Sequence analysis of S, M and ORE3 genes revealed that the central China PEDV isolates had several specific nucleotides and amino acids which were different from PEDV reference strains. In addition, the entire S genes of eleven central China PEDV isolates were found to be nine nucleotides longer in length than CV777 and large number of amino acid variations was accumulated in the N-terminal region of S gene. Phylogenetic analysis showed that the central China PEDV isolates had close relationship with Korea strains (2007-2009), Thailand strains (2007-2008), Vietnam strains (2009-2010), Japan strains (2010), and other prevailing strains from other parts of China (2010-2012). However, they differed genetically from European strains (CV777, Brl/87), China strains (2003-2007) and the vaccine strains (CV777) used in China. These results imply that a rapid variation and evolution of central China PEDV strains has occurred in recent years, and a more efficient vaccine strain should be selected to prevent and control outbreaks of PEDV in China.
Texto completo:
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Índice:
WPRIM
Asunto principal:
Filogenia
/
Porcinos
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Enfermedades de los Porcinos
/
Proteínas Virales
/
Virología
/
Datos de Secuencia Molecular
/
China
/
Epidemiología
/
Brotes de Enfermedades
/
Sistemas de Lectura Abierta
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Virology
Año:
2013
Tipo del documento:
Article