Correlation between the activation of AP-1 signal transduction pathway and metastasis of colorectal carcinoma / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 356-359, 2005.
Article
en Zh
| WPRIM
| ID: wpr-345172
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the binding activity of activator protein-1 (AP-1) with DNA probe in the colorectal carcinoma (CRC) tissues and surrounding tissues and explore the correlation between the activation of AP-1 signal transduction pathway and metastasis of CRC.</p><p><b>METHODS</b>The AP-1 DNA binding activities were investigated by electrophoretic mobility shift assay (EMSA) in CRC specimens (T), surrounding tissues including 2 cm (P(2)), 5 cm(P(5)) far away from primary tumor margin and distal resection margin of the specimens (N). The mRNA expression level of vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9) were measured by quantitive reverse transcription polymerase chain reaction (Q- RT-PCR).</p><p><b>RESULTS</b>The AP-1 DNA binding activity in T was significantly higher than those in P(2), P(5) and N (P< 0.05) tissues. There were significantly positive correlations between AP-1 DNA binding activity in tumor and invasive degree, lymphatic metastasis respectively (P< 0.01), but no correlation with histological classification and differentiation (P> 0.05). The transcription levels of VEGF and MMP-9 in CRC were significantly higher than those in P(5) and N (P< 0.01, P< 0.05) tissues. The transcription levels of VEGF and MMP-9 were significantly correlated with increasing AP-1 DNA binding activity (P< 0.01).</p><p><b>CONCLUSIONS</b>AP-1 is significantly correlated to the invasion and metastasis in CRC. The activation of AP-1 signal transduction pathway might be involved in the angiogenesis and of degradation extracellular matrix during tumor metastasis.</p>
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Índice:
WPRIM
Asunto principal:
Patología
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Neoplasias Colorrectales
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Transducción de Señal
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Factor de Transcripción AP-1
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Metaloproteinasa 9 de la Matriz
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Factor A de Crecimiento Endotelial Vascular
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Metástasis Linfática
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Metabolismo
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Estadificación de Neoplasias
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Neovascularización Patológica
Límite:
Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Gastrointestinal Surgery
Año:
2005
Tipo del documento:
Article