Small interfering RNA-mediated glucose-regulated protein 78 knockdown enhances chemosensitivity of breast cancer cells to cisplatin / 南方医科大学学报
Journal of Southern Medical University
; (12): 44-47, 2013.
Article
en Zh
| WPRIM
| ID: wpr-352311
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of small interfering RNA-mediated glucose-regulated protein 78 (GRP78) knockdown on the chemosensitivity of breast cancer cells to cisplatin.</p><p><b>METHODS</b>Human breast cancer cell line MDA-MB-231 was exposed to different doses of cisplatin (0, 1, 2, 4, 8, and 16 µmol/L), and the changes in cell viability were detected using MTT assay. PI/Annexin V staining was used to observe the apoptosis of the cells in response to transfection with a small interfering RNA targeting GRP78 (pSH1Si-GRP78). Western blotting was employed to detect GRP78 expression in pSH1Si- GRP78-transfected cells after exposure to 8 µmol/L cisplatin for 24, 48 and 72 h.</p><p><b>RESULTS</b>Exposure of the cells to 8 µmol/L cisplatin for 24, 48 and 72 h resulted in a cell survival rate of 83.13%, 54.22% and 35.79%, respectively, but the cell apoptosis rate was only 10.8% at 24 h. Transfection of MDA-MB-231 cells with pSH1Si-GRP78 caused a cell apoptosis rate of 24.6%, which increased to 48.9% in cells with both pSH1Si-GRP78 transfection and cisplatin exposure. Cisplatin exposure caused an initial up-regulation followed then by a down-regulation of GRP78 expression in MDA-MB-231 cells, while pSH1Si-GRP78 transfection produced an obvious down-regulation of GRP78 expression.</p><p><b>CONCLUSIONS</b>Inhibition of GRP78 expression increases the apoptosis and enhance cisplatin chemosensitivity of breast cancer cells in vitro, suggesting the value of GRP78 as a potential therapeutic target in the clinical treatment of breast cancer.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Farmacología
/
Transfección
/
Supervivencia Celular
/
Cisplatino
/
Apoptosis
/
ARN Interferente Pequeño
/
Línea Celular Tumoral
/
Técnicas de Silenciamiento del Gen
/
Proteínas de Choque Térmico
/
Metabolismo
Límite:
Female
/
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2013
Tipo del documento:
Article