Immune regulatory effect of human bone marrow mesenchymal stem cells on T lymphocyte / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 651-655, 2005.
Article
en Zh
| WPRIM
| ID: wpr-356495
Biblioteca responsable:
WPRO
ABSTRACT
To investigate the immune regulatory effects of human bone marrow mesenchymal stem cells on alloantigen T lymphocyte in vitro, human MSCs were isolated and expanded from bone marrow cells, and identified with cell morphology, and the phenotypes were assessed by immunohistochemistry and flow cytometry. As the stimulation factor of T lymphocytes proliferation, either PHA or dendritic cells isolated from cord blood were cocultured with CD2(+) T lymphocytes from peripheral blood mononuclear cells by magnetic beads with or without MSC in 96-well plats for seven days. T cell proliferation was assessed by [(3)H]-thymidine incorporation using a liquid scintillation counter. T cell subsets, Th1, Th2, Tc1 and Tc2 were analyzed by flow cytometry after co-culture of CD2(+) T cells with MSCs for 10 days. The results showed that a significant decrease of CD2(+) T cell proliferation was evident when MSC were added back to T cells stimulated by DC or PHA, and an increase of Th2 and Tc2 subsets were observed after co-culture of MSC with T lymphocytes. It is suggested that allogeneic MSC can suppress T cell proliferation in vitro and the cause of that was partly depend on interaction of cells and the alteration of T cell subsets.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Células de la Médula Ósea
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Inmunohistoquímica
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Linfocitos T
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Comunicación Celular
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Células Cultivadas
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Subgrupos de Linfocitos T
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Antígenos CD2
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Técnicas de Cocultivo
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Biología Celular
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Proliferación Celular
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2005
Tipo del documento:
Article