Reversal of multidrug resistance of HL-60 adriamycin resistant leukemia cell line by quercetin and its mechanisms / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 326-329, 2005.
Article
en Zh
| WPRIM
| ID: wpr-358640
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Quercetin, a widely distributed natural flavonoid with a variety of biological functions, can reverse multidrug resistance (MDR) in leukemia according to recent researches. The aim of this study was to investigate the mechanisms of reversal of multi-drug resistance by quercetin mainly in respect of membrane transporters.</p><p><b>METHODS</b>MTT cell viability assay was used to verify the chemo-sensitization to daunorubicin (DNR) by quercetin in HL-60/ADM cell line and determine the effective reversal concentration, the expression of MRP(1) gene and its protein product, multidrug resistant associated protein 1 by RT-PCR and flow cytometry By confocal laser scanning microscopy, the subcellular distribution of DNR in HL-60/S and HL-60/ADM cells was examined before and after quercetin exposure.</p><p><b>RESULTS</b>Compared with HL-60/S, 20-40 micromol/L quercetin in vitro remarkably enhanced the sensitivity of HL-60/ADM cells to daunorubicin, down-regulated the expression of MRP(1) gene and its protein product MRP(1), restored the abnormal subcellular distribution of daunorubicin, so as to reverse MDR. Moreover, such an effective concentration of quercetin was non-toxic to the cells.</p><p><b>CONCLUSION</b>Quercetin could be a candidate of effective multidrug resistance-reversing agent with low toxicity in leukemia chemotherapy.</p>
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Índice:
WPRIM
Asunto principal:
Farmacología
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Quercetina
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Daunorrubicina
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Miembro 1 de la Subfamilia B de Casetes de Unión a ATP
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Resistencia a Múltiples Medicamentos
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Células HL-60
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Resistencia a Antineoplásicos
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Subfamilia B de Transportador de Casetes de Unión a ATP
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Antibióticos Antineoplásicos
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Año:
2005
Tipo del documento:
Article