Soy isoflavone and its effect to regulate hypothalamus and peripheral orexigenic gene expression in ovariectomized rats fed on a high-fat diet / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 68-75, 2010.
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| ID: wpr-360621
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ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of soy isoflavone on obesity in the light of hypothalamus and peripheral orexigenic gene regulation.</p><p><b>METHODS</b>Fifty-four female rats were randomly assigned to 6 groups: one sham-operated group (SHAM), one ovariectomized (OVX) control group, three OVX groups fed with 400 ppm (L-SI), 1200 ppm (M-SI) and 3600 ppm (H-SI) isoflavone respectively, and one OVX group receiving 0.45 ppm diethylstilbestrol (EC). All rats were allowed to take high-fat diet for 4 weeks. Some neuropeptides were measured by RT-PCR. These neuropeptides included NPY, pro-opiomelanocortin (POMC), cocaine and amphetamine regulated transcript (CART), orexin, melanin-concentrating hormone (MCH), melanin-concentrating hormone precursor (P-MCH), ghrelin, and leptin.</p><p><b>RESULTS</b>Compared with the OVX control group, the body weight and food intake in the H-SI group were reduced significantly and there was a significant dose-dependent manner in the 3 isoflavone groups. The results of RT-PCR showed that the NPY level in the 3 isoflavone groups was significantly increased and the POMC/CART gene expression decreased significantly in rats' hypothalamus compared with that in the OVX control group. However, the expression of orexin, MCH and P-MCH had no change. The peripheral grelin mRNA expression was higher in the 3 isoflavone groups, while leptin gene expression in the fat was not consistent.</p><p><b>CONCLUSIONS</b>This research showed that isoflavone could prevent obesity induced by high-fat diet and ovariectomy through regulating hypothalamus and peripheral orexigenic gene expressions associated with food intake.</p>
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Asunto principal:
Farmacología
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Fisiología
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Glycine max
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Neuropéptidos
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ARN Mensajero
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Grasas de la Dieta
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Ovariectomía
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Química
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Regulación de la Expresión Génica
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Conducta Alimentaria
Límite:
Animals
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Año:
2010
Tipo del documento:
Article