Fermentation research of new type recombinant human tumor necrosis factor alpha / 中国生化药物杂志

ABSTRACT

Purpose　The aim is to establish fermentation process of new type recombinant human necrosis factor (nrhTNF)α.Methods　The optimized host cell,culture medium and induction time were deternined according to the growth and expression specificity of nrhTNF on test tube and flask shaker. Then fed-batch culture was carried out on 5 L automatic fermentor.Results　The expression of nrhTNF in fermentor could keep in a higher level of about 50% of total bacterial proteins when fermented to around A600nm 30，with a fermentation time of 12 h to 13 h.Conclusion　A short cycle,high expression and stabilized fermentation process was established.