Role of circulating microRNA-22 in pathogenesis of idiopathic pulmonary arterial hypertension / 中华急诊医学杂志
Chinese Journal of Emergency Medicine
; (12): 57-60, 2012.
Article
en Zh
| WPRIM
| ID: wpr-424442
Biblioteca responsable:
WPRO
ABSTRACT
Objective To investigate the value of plasma miR-22 in diagnosis of idiopathic pulmonary arterial hypertension ( IPAH ),and its role of regulation mechanisms in the pathogenesis of the disease.Methods Circulating miR-22 levels of IPAH patients and healthy controls were evaluated by RTPCR.The silico analysis of targets for miR-22 was taken, and followed by eGFP reporter assay for verification of predicted target gene Myc binding protein (MYCBP). Results Compared with healthy controls,the expression of plasma miR-22 in IPAH patients was significantly decreased (P < 0.01 ).The area under curve (AUC) of ROC curve was 0.744.MYCBP was a real target of miR-22 confirmed by silico analysis and eGFP reporter assay. Conclusions The expression of plasma miR-22 was significantly decreased in IPAH patients,and it could serve as a potential biomarker for diagnosis.The miR-22 might be involved in the pathogenesis of the disease through promoting its target gene MYCBP to activate the c-Myc pathway.
Texto completo:
1
Índice:
WPRIM
Tipo de estudio:
Etiology_studies
/
Prognostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Emergency Medicine
Año:
2012
Tipo del documento:
Article