The effect of fluoride on the viability of rat ameloblast HAT-7 cells and Ca2 + concentration in the cells / 实用口腔医学杂志
Journal of Practical Stomatology
; (6): 7-10, 2015.
Article
en Zh
| WPRIM
| ID: wpr-462136
Biblioteca responsable:
WPRO
ABSTRACT
Objective:
To evaluate the effect of fluoride on the viability of rat ameloblast HAT-7 cells and calcium concentration in the cells.Methods:
HAT-7 cells were exposed to NaF at 0,0.4,0.8,1.6,3.2 and 6.4 mmol/L for 24,48 and 72 h respectively. CCK-8 assay was performed to examine the cells proliferation;the apoptosis rate was determined by flow cytometry;Ca2 +concentration in the cells was detected by laser scanning confocal microscopy.Results:
The cell proliferation was increased by NaF at 0.4 mmol/L and 0.8 mmol/L,whereas inhibited at 1.6 mmol/L and above.The effects were in a time-dependent manner.NaF increased apoptosis of the cells and increased Ca2 + concentration in the cells in a concentration-dependent manner.Conclusion:
Fluoride at low doses promotes proliferation,at high doses inhibits proliferation of HAT-7 cells.NaF of 1.6 mmol/L or more induces apoptosis of HAT-7 cells and in-duce Ca2 + overloading in the cells.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Journal of Practical Stomatology
Año:
2015
Tipo del documento:
Article