Effect of persistent CO_2-insufflation on mRNA expression of adhesion molecules of colon cancer cell lines / 中国普通外科杂志
Chinese Journal of General Surgery
; (12)2000.
Article
en Zh
| WPRIM
| ID: wpr-526075
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WPRO
ABSTRACT
Objective To investigate the effect of CO_2-insufflation on mRNA expression of adhesion molecules of colorectal cancer cell lines. Methods With an artificial pneumoperitoneum model in vitro, SW1116 human colon carcinoma cell lines were exposed to CO_2-insufflation of different pressure ( 6mmHg , 9mmHg, 12mmHg and 15mmHg) for 1 hour. The mRNA level of E-cadherin, ICAM-1, CD44 and CD44v6 were measured after 0, 12, 24, 48 and 72 hours by real-time RT-PCR. Control cell lines were exposed to room air. ResultsThe mRNA expression of E-cadherin, ICAM-1, CD44 and CD44v6 increased significantly at 0h~48h after the cells were exposed to the continuous CO_2-insufflation for 1 hour(P
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WPRIM
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Zh
Revista:
Chinese Journal of General Surgery
Año:
2000
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Article