Impact of HBV DNA level in serum on the cloning efficiency of HBV full length genome / 中国病理生理杂志

ABSTRACT

AIM:To compare the cloning efficacy of full-length HBV genome amplified by single fragment PCR and two fragment PCR for choosing the suitable method for full-length HBV genome cloning.METHODS:To amplify the full-length HBV genome from 85 sera sample of HBV patients,single fragment PCR and two fragment PCR were conducted.The products were cloned into the vector and sequenced after identified with double enzyme digestion.At the same time,the titers of 85 samples were detected by real-time PCR.RESULTS:Compared with two fragment PCR,single fragment PCR requested higher level of sera HBV DNA for successful amplification of full-length HBV genome,and the efficacy of single fragment PCR was lower than that of two fragments PCR(P