Ischemia reperfusion-induced proteomic changes in rat skeletal muscle / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)2000.
Article
en Zh
| WPRIM
| ID: wpr-531403
Biblioteca responsable:
WPRO
ABSTRACT
AIM:
To investigate ischemia reperfusion (I/R)-induced proteomic changes in rat skeletal muscle.METHODS:
Healthy male Wistar rats were randomly divided into two groups as follows (n=6) sham group and I/R group. I/R of right hind limb was induced by 4 h ischemia followed by 24 h reperfusion. The 2-DE was applied to separate the proteins extracted from skeletal muscle tissue at the end of experiment, followed by Coomassie Brillant blue R-250 staining. Computer image analysis was used to determine the differential expression of proteins between the two groups, and 7 protein spots expressed differentially were picked out and subjected to in-gel digest and MALDI-TOP for identification.RESULTS:
354?13 proteins were detected and the match rate was (78.7?1.4)%. 10 proteins displayed significant changes after I/R, of which, 6 proteins increased and 3 proteins decreased in expression. Moreover, 2 spots in I/R group were observed, only 1 spots of which in control. 5 proteins were identified after mass spectrometry. Mitochondrial aldehyde dehydrogenase (ALDH) precursor, heat shock 27 kD protein (HSP27), an unnamed protein product (increased in I/R group), ?-actin (decreased in I/R group), and nuclear transport factor 2 (NTF-2) W7a mutant were found in I/R group.CONCLUSION:
I/R injury induced differential proteomic changes in rat skeletal muscle. ALDH, ?-actin and HSP27 expression, and NTF-2 mutation are involved in I/R injury.
Texto completo:
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Índice:
WPRIM
Tipo de estudio:
Prognostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Pathophysiology
Año:
2000
Tipo del documento:
Article