A preliminary study on the expression of recombinant plasmid PcDNA3.1/sTNFRⅠ in mice / 实用口腔医学杂志

ABSTRACT

Objective:To explore whether the recombinant plasmid PcDNA3.1/ sTNFRⅠcould be expressed properly in mice.Methods:The recombinant plasmid PcDNA3.1/sTNFRⅠand the empty vector PcDNA3.1 were directly injected into the right tibialis anterior muscle of each 6 mice respectively. The expression product, sTNFRⅠ, in muscle homogenates was measured by ELISA 7 and 10 days after injection respectively.Results:7 days after injection sTNFR Ⅰ(mg/g) in muscle homogenates in PcDNA3.1/sTNFRⅠ and PcDNA3.1 injected mice was 161.123?4.0 and 69.34?1.489(P