Hydrogen sulfide inhibited expression of elastin in pulmonary arteries of hypoxic pulmonary hypertensive rats / 中国药理学通报

ABSTRACT

Aim To investigate the inhibitory effect of hydrogen sulfide on expression of elastin in hypoxic pulmonary hypertensive (HPH) rats. Methods Twenty-four rats were randomly divided into three groupscontrol group (n=8), hypoxic group (n=8) and hypoxia+NaHS group (n=8). Pulmonary artery mean pressure (mPAP) of three group rats was measured via right ventricular catheterization. Right ventricle and left ventricle+septum of each rats were isolated from intact rats and weight of right ventricle/(weight of left ventricle+septum) ratio was calculated. The percentage of muscularized arteries of small pulmonary arteries was measured using a light microscope. The expression of elastin and TGF-? in pulmonary artery smooth muscle cells was observed with immunohistochemistry. Results mPAP and weight of right ventricle/(weight of left ventricle+septum) ratio were significantly higher in rats of hypoxic group than those of control group (P