Determination of Chlorogenic Acid in Yinren Granules by HPLC / 中药新药与临床药理
Article
en Zh
| WPRIM
| ID: wpr-573508
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WPRO
ABSTRACT
Objective To establish a method for the content determination of c hlorogenic acid in Yinren Granules. Methods The content of chlorogenic acid wa s detected by HPLC. HPLC was performed with Shim- Pack CLC- ODS column(150? 6.0 mm, 5 ? m) at room temperature, 3 % methyl alcohol- methanol( 79 ∶ 2 1) as mobile phase, the detection wavelength at 327 nm, the flow rate being 1. 2 mL/min and sample loop volume being 10 ? L. Results Chlorogenic acid has a good linearity within 0.102~ 1.02? g (r=0.999)and the average recovery was 98. 17 % with RSD being 1.31 % . Conclusion This method is simple, accurate and can be used for the quality control of Yinren Granules.
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WPRIM
Idioma:
Zh
Revista:
Traditional Chinese Drug Research & Clinical Pharmacology
Año:
1993
Tipo del documento:
Article