Expression and Significance of Fas and FasL in Hyperthyroid Cardiopathy / 重庆医科大学学报

ABSTRACT

Objective:To study the expression of Fas and FasL in hyperthyroid cardiopathy and to explore its possible effect and clinical significance in the pathogenesis of the hyperthyroid cardiopathy.Methods:Twenty New Zealand rabbits were randomly divided into two groups control group(n =10) and experimental group(n =10).Hyperthyroidism animal model was established by peritoneal injection of Levothyroxine(L-Thy) in experimental group.All the rabbits were followed up for 4 weeks.Every 7 days blood samples were obtained.Serum TT3 and TT4 were measured with radioimmunoassays(RIAs).Electrocardiogram(ECG) was performed to determine heart rate(HR).All the rabbits underwent echocardiography and the thickness of IVS?LV?LA?LVPW and LVEF were measured at the end of the follow-up period.Ventricular tissues were collected at 4th weeks.Cardiac hypertrophy index,cardiomyocyte diameter,structural change were detected.Cardiac fibrosis was displayed by Masson stain and collagen volume fraction(CVF)was measured using pathological image analytic system.Protein of Fas and FasL were localized by immunohistochemistry and semi-quantified with pathological image analytic system.Results: In experimental group,the thickness of IVS?LV?LA?LVPW,cardiac quality index,cardiomyocyte diameter,collagen volume fraction(CVF) and the expression of Fas in left atrium and left ventricle increased obviously(P