Influence of Bufalin on the Growth and Proliferation of Human Glioma Cell Line U251 / 中国药师
China Pharmacist
; (12): 825-827,828, 2016.
Article
en Zh
| WPRIM
| ID: wpr-604252
Biblioteca responsable:
WPRO
ABSTRACT
Objective:
To explore the effects of Bufalin on the growth and proliferation of human glioma cells U251.Methods:
Methyl thiazolyl tetrazolium(MTT)assay was used to detect the effect of Bufalin on the proliferation of human glioma cells U251. An in-verted microscope was used to observe the changes of cell number,morphology and activity. AnnexinV/ PI was used to measure the in-duction of cell apoptosis caused by Bufalin.Results:
Bufalin at different concentrations(0. 001 - 10. 0μmol·L - 1 )inhibited the pro-liferation of U251 cells in a dose and time-dependent manner. Compared with that of the control group,the apoptosis rate of Bufalin group was increased significantly(P < 0. 01).Conclusion:
Bufalin can inhibit the growth and proliferation of U251 cells in a dose and time-dependent manner,and induce the apoptosis of U251 cells.
Texto completo:
1
Índice:
WPRIM
Idioma:
Zh
Revista:
China Pharmacist
Año:
2016
Tipo del documento:
Article