Transcriptional Regulation of the Xbr-1a/Xvent-2 Gene by BMP-4 Signaling during Xenopus Embryonic Development / 대한해부학회지
Korean Journal of Anatomy
; : 595-608, 2000.
Article
en Ko
| WPRIM
| ID: wpr-653755
Biblioteca responsable:
WPRO
ABSTRACT
BMP-4 signaling is mediated through Smad proteins which may translocate to the nucleus to activate transcription. Little is known about how BMP-4 signaling regulates the transcription of its target genes, e.g., Xvent genes. Therefore, we isolated the genomic clone of a BMP-4 responsive homeobox gene, Xbr-1a/Xvent-2. This clone contains a promoter and three exons for the entire coding region. Using the primer extension, we identified the transcription initiation site corresponding to position -64 bp upstream to the ATG codon of the Xvent-2 gene. The promoter was linked to the luciferase reporter gene, and promoter activity determined by luciferase assay. The temporal promoter activity peaked between embryonic stages 13~17, in agreement with its temporal mRNA expression in the whole embryo. Through the serial deletion mutation, the upstream -235 bp of the promoter retains the full transcriptional activity, and is regulated by BMP-4 signaling. The present results suggest that the BMP-4 responsive element is located on the upstream 235 bp of the promoter.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Xenopus
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Xenopus laevis
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Codón
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ARN Mensajero
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Genes Homeobox
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Exones
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Células Clonales
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Eliminación de Secuencia
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Genes Reporteros
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Sitio de Iniciación de la Transcripción
Límite:
Pregnancy
Idioma:
Ko
Revista:
Korean Journal of Anatomy
Año:
2000
Tipo del documento:
Article