Study on HPLC Fingerprint of Zhenqi Fuzheng Granules / 中国药房
China Pharmacy
; (12): 4691-4694, 2017.
Article
en Zh
| WPRIM
| ID: wpr-668659
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE:
To establish HPLC fingerprint of Zhenqi fuzheng granules.METHODS:
HPLC-ELSD method was ad-opted. The determination was performed on Agilent Zorbax Eclipse XDB-C18 column with mobile phase consisted of acetonitrile-wa-ter(gradient elution)at the flow rate of 1.0 mL/min with column temperature of 40 ℃. The detector evaporation temperature was 50℃,and sample size was 10μL. Using specnuezhenide as reference,HPLC chromatograms of 7 batches of Zhenqi fuzheng gran-ules were determined. The identification and similarity evaluation of common peaks were conducted by using the TCM Chromato-graphic Fingerprint Similarity Evaluation System(2004 A edition).RESULTS:
There were 13 common peaks in HPLC chromato-grams of 7 batches of samples. After validated,the similarity of 3 batches of samples in HPLC chromatograms of 7 batches of sam-ples were higher than 0.9,which were in good agreement with control fingerprints. The similarity of 4 batches of samples were <0.9,which were poorly same to control fingerprint.CONCLUSIONS:
Established fingerprint can provide reference for quality evalu-ation of Zhenqi fuzheng granules.
Texto completo:
1
Índice:
WPRIM
Idioma:
Zh
Revista:
China Pharmacy
Año:
2017
Tipo del documento:
Article