Effect of escharectomy on rats'pulmonary NF-?B activation in early stage of burn injury / 中华急诊医学杂志
Chinese Journal of Emergency Medicine
; (12)2006.
Article
en Zh
| WPRIM
| ID: wpr-683077
Biblioteca responsable:
WPRO
ABSTRACT
Objective To investigate the effect of escharectomy on rats' pulmonary NF-?B activation and the expression of pulmonary proinflammatory cytokines in early stage of burn injury.Method Wistar rats were randomly divided into three groups:group A(control group),group B(postburn without escharectomy),group C(escharectomy at early stage of burn injury).Thermal-injuried rats underwent 35% TBSA full-thickness burns. Activation of pulmonary NF-?B at 12 hours and 24 hours postburn was tested by electrophoretic mobility shift assay (EMSA),and at the same time expressions of pulmonary TNF-?mRNA were measured by reverse transcription polymerase chain reaction(RT-PCR)and release of pulmonary TNF-?were assayed by enzyme-linked immunosorbent assay(ELISA).Results Compared with control group,activity of pulmonary NF-?B in group B was markedly increased,reached(19.56?1.36)?10~4 A at 12 hours and(15.23?1.94)?10~4 A at 24 hours,which was higher than that in group A[(4.36?0.38)?10~4 A,P
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Emergency Medicine
Año:
2006
Tipo del documento:
Article