ADAR1 up-regulates ZNF655 expression via RNA editing and enhances HBV replication in HepG2 cell line / 基础医学与临床

ABSTRACT

Objective To explore the effect of ADAR1 on ZNF655 and the regulation of ZNF655 on the expression of HBV. Methods Sanger sequencing was used to validate the 3′UTR region of ZNF655 in ADAR1. The expression of ADAR1 and ZNF655 mRNA as well as HBV RNA were detected by RT-qPCR. Dual luciferase report plasmid assay was used to detect the expression of luciferase. To detect the expression of ADAR1 and ZNF655 pro-tein by Western blot. HBsAg and HBeAg was detected by ELISA. Results The chr7:99575277 loci on ZNF655 3′UTR was homozygous in DNA level and hybrid in RNA level. On the 3′UTR editing site of ZNF655,the luciferase activity of the edited G allele was significantly higher than that of the normal A allele (P＜0.001). The expression of ZNF655 was upregulated by ADAR1 in the level of transcription and translation(P＜0.01).ZNF655 significantly promoted the expression of HBV. Conclusions The chr7:99575277 loci on ZNF655 3′UTR is edited by ADAR1, promoting the expression of ZNF655,which upregulated the expression of HBV.