Expression and significance of CD68, E-cadherin and TGF-β1 in breast cancer / 中华内分泌外科杂志

ABSTRACT

Objective To investigate the expression and significance of CD68,E-cadherin and transforming growth factor-β1 (TGF-β1) in breast cancer.Methods Immunohistochemistry was used to detect the expression of CD68,E-cadherin and TGF-β1 in breast cancer tissues.The relationship between the expression of proteins with the clinicopathological parameters and the recurrence of breast cancer was analyzed.And the correlation among the three proteins was also analyzed.Results The positive expression rate of CD68 and E-cadherin was 70.4％ and 72.6％,respectively,which was significantly higher than that in adjacent tissues(CD68:x2=44.278,P=0.000;TGF-β1:x2=121.529,P=0.000).The positive expression rate of E-cadherin in breast cancer was 29.1％,which was significantly lower than that in adjacent tissues(x2=244.965,P=0.000).The expression of CD68 protein was closely related to clinical stage (x2=11.720,P=0.003),lymph node metastasis (x2=9.394,P=0.002) and ER status (x2=5.204,P=0.023).The expression of E-cadherin protein was significantly correlated with the histological grade(x2=6.561,P=0.038) and lymph node metastasis(x2=6.892,P=0.009),and the expression of TGF-β1 protein was significantly correlated with the histological grade(x2=6.427,P=0.040) and ER status(x2=5.755,P=0.016) of patients.The three proteins were all significantly related to the 5 year recurrence of breast cancer (CD68:γ=0.152,P=0.021;TGF-β1:γ=0.157,P=0.017:E-cadherin:γ=-0.145,P=0.028).The expression of CD68 protein was negatively correlated with E-cadherin protein (γ=-0.151,P=0.022),and positively correlated with the expression of TGF-[β1 (γ=0.200,P=0.002).E-cadherin protein was negatively correlated with the expression of TGF-β1 protein(γ=-0.143,P=0.031).Conclusion CD68 positive cells (tumor associated macrophages,TAMs),E-cadherin and TGF-β1 may be involved in the progression of breast cancer,detection of the expression will provide the theoretical basis and guidance for the clinical pathological diagnosis and treatment of breast cancer.