Comparative study of oxidative stress caused by anthracene and alkyl-anthracenes in Caenorhabditis elegans
Environmental Health and Toxicology
; : 2018006-2018.
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| ID: wpr-786739
Biblioteca responsable:
WPRO
ABSTRACT
Oxidative stress was evaluated for anthracene (Ant) and alkyl-Ants (9-methylanthracene [9-MA] and 9,10-dimethylanthracene [9,10-DMA]) in Caenorhabditis elegans to compare changes in toxicity due to the degree of alkylation. Worms were exposed at 1) the same external exposure concentration and 2) the maximum water-soluble concentration. Formation of reactive oxygen species, superoxide dismutase activity, total glutathione concentration, and lipid peroxidation were determined under constant exposure conditions using passive dosing. The expression of oxidative stress-related genes (daf-2, sir-2.1, daf-16, sod-1, sod-2, sod-3 and cytochrome 35A/C family genes) was also investigated to identify and compare changes in the genetic responses of C. elegans exposed to Ant and alkyl-Ant. At the same external concentration, 9,10-DMA induced the greatest oxidative stress, as evidenced by all indicators, except for lipid peroxidation, followed by 9-MA and Ant. Interestingly, 9,10-DMA led to greater oxidative stress than 9-MA and Ant when worms were exposed to the maximum water-soluble concentration, although the maximum water-soluble concentration of 9,10-DMA is the lowest. Increased oxidative stress by alkyl-Ants would be attributed to higher lipid-water partition coefficient and the π electron density in aromatic rings by alkyl substitution, although this supposition requires further confirmation.
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Asunto principal:
Hormigas
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Hidrocarburos Policíclicos Aromáticos
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Superóxido Dismutasa
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Caenorhabditis
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Peroxidación de Lípido
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Expresión Génica
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Especies Reactivas de Oxígeno
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Caenorhabditis elegans
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Estrés Oxidativo
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Citocromos
Límite:
Humans
Idioma:
En
Revista:
Environmental Health and Toxicology
Año:
2018
Tipo del documento:
Article