Function of miR-100 on hepatocellular carcinoma invasion and metastasis / 中华肝胆外科杂志
Chinese Journal of Hepatobiliary Surgery
; (12): 725-727, 2019.
Article
en Zh
| WPRIM
| ID: wpr-791490
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WPRO
ABSTRACT
Objective To study the expression of microRNA-100 ( miR-100 ) in hepatocellular carcinoma ( HCC) tissues and cells, and further explore the function of miR-100 on invasion and metastasis of HCC. Methods A total of 50 patients with HCC from December 2016 to October 2018 in Department of Hepatobiliary Pancreatic Surgery, Henan Provincial People's Hospital were selected, including 32 males and 18 females, aged 20~78 years. PCR was used to detect the expression of miR-100 in HCC tissues, adjacent tissues and HCC cells. HCC cells were transfected with miR-100 sequence, inhibition sequence and corre-sponding negative control sequence to detect the migration and invasion ability of HCC cells after transfec-tion. Results The expression of miR-100 was lowest in SMMC-7721 and MHCC-97H. The relative expres-sion level of miR-100 in HCC tissues was significantly lower than that in normal adjacent liver tissues [(0. 39 ± 0. 03) vs. (0. 56 ± 0. 06)], and the difference was statistically significant (P<0. 05). Compared with the negative control HCC cells, the migration and invasion of HCC cells with overexpression of miR-100 decreased, while the inhibition of miR-100 expression increased the migration and invasion of HCC cells. Conclusions The expression of miR-100 is low in human HCC tissues and cells. miR-100 inhibits the invasion and migration of HCC cells and plays an important role in the occurrence and development of HCC.
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Zh
Revista:
Chinese Journal of Hepatobiliary Surgery
Año:
2019
Tipo del documento:
Article