Effects of apigenin on self-renewal and
uPAR expression in NCI-H446 cell line / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 1124-1127, 2016.
Article
en Zh
| WPRIM
| ID: wpr-815122
Biblioteca responsable:
WPRO
ABSTRACT
To investigate the effect of apigenin on self-renewal for sphere-forming cells in human small cell lung cancer cell line NCI-H446 and the underlying mechanisms.
Methods: Sphere-forming cells from NCI-H446 cell line were cultured in stem cell-conditioned culture medium with ultra-low attachment surface plates. The rate of sphere-forming cells in the second passage sphere-forming cells was used to evaluate the inhibitory effects of apigenin on the self-renewal for sphere-forming cells. The protein level of urokinase-type plasminogen activator receptor (uPAR) in spheroids was analyzed by Western blot.
Results: Apigenin signifcantly inhibited the self-renewal of the second passage sphere-forming cells [0, 5.0, 10.0, 20.0 μmol/L apigenin: (18.2±1.9)%, (13.6±1.7)%, (10.6±1.6)%, (6.9±1.3)%, respectively] and down-regulated uPAR expression in a concentration-dependent manner (P<0.05).
Conclusion: Apigenin inhibits the self-renewal capacity of sphere-forming cells in NCI-H446 cells, which may be associated with down-regulation of uPAR expression.
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Índice:
WPRIM
Asunto principal:
Patología
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Farmacología
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Fisiología
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Células Madre
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Células Madre Neoplásicas
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Transducción de Señal
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Regulación hacia Abajo
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Receptores de Superficie Celular
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Esferoides Celulares
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Línea Celular Tumoral
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Año:
2016
Tipo del documento:
Article