beta-Glucan enhanced apoptosis in human colon cancer cells SNU-C4
Nutrition Research and Practice
; : 180-184, 2009.
Article
en En
| WPRIM
| ID: wpr-81756
Biblioteca responsable:
WPRO
ABSTRACT
The apoptotic effect of bacteria-derived beta-glucan was investigated in human colon cancer cells SNU-C4 using terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR) expressions of Bcl-2, Bax, and Caspase-3 genes, and assay of caspase-3 enzyme activity. beta-Glucan of 10, 50, and 100 microg/mL decreased cell viability in a dose-dependent manner with typical apoptotic characteristics, such as morphological changes of chromatin condensation and apoptotic body formation from TUNEL assay. In addition, beta-glucan (100 microgram/mL) decreased the expression of Bcl-2 by 0.6 times, whereas the expression of Bax and Caspase-3 were increased by 3.1 and 2.3 times, respectively, compared to untreated control group. Furthermore, the caspase-3 activity in the beta-glucan-treated group was significantly increased compared to those in control group (P < 0.05). Bacterial derived beta-glucan could be used as an effective compound inducing apoptosis in human colon cancer.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Cromatina
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Supervivencia Celular
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Apoptosis
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Colon
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Neoplasias del Colon
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Etiquetado Corte-Fin in Situ
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ADN Nucleotidilexotransferasa
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Caspasa 3
Límite:
Humans
Idioma:
En
Revista:
Nutrition Research and Practice
Año:
2009
Tipo del documento:
Article