Differences of intestinal flora between gestational diabetes mellitus pregnant women and healthy pregnant women / 上海交通大学学报（医学版）

ABSTRACT

Objective • To investigate the differences of intestinal flora between gestational diabetes mellitus (GDM) pregnant women and healthy pregnant women. Methods • In this study, 74 pregnant women from the Department of Obstetrics in Xinhua Hospital, Shanghai Jiao Tong University School of Medicine from Aug. 2017 to Jan. 2019 were recruited, including 51 GDM pregnant women (GDM group) and 23 healthy pregnant women (healthy group). High-throughput sequencing was performed on bacterial gene sequences from the fresh feces of all pregnant women by using Illumina MiSeq platform. Linear discriminant analysis (LDA) and LDA EffectSize (LEfSe) were used to detect the α diversity and expression abundance of intestinal flora of the two groups. Results • Compared with the healthy group, the intestinal flora richness of pregnant women in the GDM group was significantly higher (P=0.027). Porphyromonadaceae and Alcaligenaceae in Family, and Bacteroides in Genus of microbiota in the GDM group were significantly higher (all P<0.05). Compared with the GDM group, Burkholderiales, Lactobacillales and Bacillales in Order, Lachnospirace in Family, and Neisseria, Streptococcus, Butyricicoccusm, Gardnerella and Atopobium in Genus of microbiota in the healthy group were significantly higher (all P<0.05). Conclusion • There are significant differences of intestinal flora between GDM pregnant women and healthy pregnant women.