A new macamide from Lepidium meyenii cultivated in Lijiang / 中草药

ABSTRACT

Objective To study the chemical constituents of the dried roots of Lepidium meyenii cultivated in Lijiang. Methods The samples were extracted by 90% alcohol, and then isolated by silica column, MCI, and HPLC. The structures of the isolated compounds were elucidated by NMR techniques including 2D NMR such as HSQC, HMBC, and COSY. The cytotoxic activity of the new compound against five cell lines (NB4, A549, PC3, SHSY5Y, and MCF7) was evaluated by MTT methods. Results Three macamides were isolated and identified as N-(3,4-dimethoxybenzyl) hexadec-9Z-enamide (1), N-benzyl-13-oxo-9E,11E-octadecadienamide (2) and N-(3,4-dimethoxybenzyl)-hexadecanamide (3) from this plant. Conclusion Compound 1 is a new compound named macalepidiumide A and shows no significant cytotoxic activity.