Identification of metabolites of pulsatilla saponin B7 in rats and in vitro by UHPLC-Q-TOF-MS / 中国药学杂志
Chinese Pharmaceutical Journal
; (24): 1204-1211, 2016.
Article
en Zh
| WPRIM
| ID: wpr-859040
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE:
To identify the metabolites oi Pulsatilla oleanolic acid 3-α-L-rhamnopyranosyl (1→2) [β-D-glucopyr-anosyl-(1→4)]-α-L-pyran arabinoside (B7) in rat plasma, bile, urine, feces, and in vitro liver microsomes incubation system.METHODS:
An ultra-high performance liquid chromatography combined with electrospray ionization quadrupole time of flight mass spectrometry (UHPLC-Q-TOF-MS) method was developed and successfully applied to the study on the metabolites of B7 in rat plasma, bile, urine, feces, and in vitro liver microsomes incubation system after oral administration.RESULTS:
A total of 23 metabolites were identified, of which 12 metabolites were present in rat liver microsomes incubation system.CONCLUSION:
Deglycosylation, demethylation glucuronidation, and hydroxylation are the major metabolic transformation forms of B7 in rats in vivo and in vitro.
Texto completo:
1
Índice:
WPRIM
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
Chinese Pharmaceutical Journal
Año:
2016
Tipo del documento:
Article