CRISPR/Cas9 technology in disease research and therapy: a review / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1205-1228, 2021.
Article
en Zh
| WPRIM
| ID: wpr-878625
Biblioteca responsable:
WPRO
ABSTRACT
Genome editing is a genetic manipulation technique that can modify DNA sequences at the genome level, including insertion, knockout, replacement and point mutation of specific DNA fragments. The ultimate principle of genome editing technology relying on engineered nucleases is to generate double-stranded DNA breaks at specific locations in genome and then repair them through non-homologous end joining or homologous recombination. With the intensive study of these nucleases, genome editing technology develops rapidly. The most used nucleases include meganucleases, zinc finger nucleases, transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeats associated Cas proteins. Based on introducing the development and principles of above mentioned genome editing technologies, we review the research progress of CRISPR/Cas9 system in the application fields of identification of gene function, establishment of disease model, gene therapy, immunotherapy and its prospect.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Tecnología
/
Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas
/
Sistemas CRISPR-Cas
/
Nucleasas de los Efectores Tipo Activadores de la Transcripción
/
Edición Génica
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2021
Tipo del documento:
Article