Role of autophagy in lipopolysaccharide-induced apoptosis of odontoblasts / 南方医科大学学报
Journal of Southern Medical University
; (12): 1816-1820, 2020.
Article
en Zh
| WPRIM
| ID: wpr-880806
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the role of autophagy in lipopolysaccharide (LPS)-induced apoptosis of murine odontoblasts.@*METHODS@#Murine odontoblasts (mDPC-23 cells) were treated with 5 μg/mL LPS for 6, 12 and 24 h, and the changes in cell viability was examined using CCK8 kit and cell apoptosis was detected by TUNEL staining. The changes in the protein levels of LC3, Beclin1, Atg5, AKT, p-AKT, mTOR and p-mTOR were detected using Western blotting. The effect of 3-MA treatment for 24 h on LPS-induced apoptosis of mDPC-23 cells was evaluated by detecting the expressions of apoptosis-related proteins caspase-3 and Bax using Western blotting.@*RESULTS@#Stimulation with LPS for 6 and 12 h did not cause significant changes in the proliferation or apoptosis of mDPC-23 cells, but LPS treatment for 24 h significantly suppressed cell proliferation (@*CONCLUSIONS@#LPS stimulation induces autophagy to promote apoptosis of mDPC-23 cells, and suppression of autophagy attenuates LPS-induced apoptosis. Autophagy may play an important role in the injury of inflamed pulp tissues.
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Índice:
WPRIM
Asunto principal:
Autofagia
/
Transducción de Señal
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Lipopolisacáridos
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Apoptosis
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Proteínas Proto-Oncogénicas c-akt
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Odontoblastos
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2020
Tipo del documento:
Article