Usefulness of the outer membrane proteins of Shigella sonnei in developing an antibody-based immunoassay for the diagnosis of shigellosis
Tropical Biomedicine
; : 252-256, 2022.
Article
en En
| WPRIM
| ID: wpr-937026
Biblioteca responsable:
WPRO
ABSTRACT
@#Conventional culture method and biochemical tests remain as the ‘gold standard’ method for the identification of S. sonnei which are time-consuming. We have discovered previously the potential of three OMPs of S. sonnei (33.3 kDa, 43.8 kDa and 100.3 kDa) as biomarkers in the diagnostic test for shigellosis. Here, we evaluated the performance of the outer membrane proteins of S. sonnei for the development of an antibody-based immunoassay for the detection of S. sonnei infections. All threetarget proteins were specifically recognized when probed with S. sonnei sera. In addition, another two potential proteins of molecular weight 29.0 kDa and 88.2 kDa in size were also exclusively recognized by the IgA when probed with S. sonnei sera. The optimized ELISA demonstrated higher sensitivity and specificity which exceeded 86.0%. In conclusion, the identified target proteins showed great potential as diagnostic biomarkers for the detection of S. sonnei infections in patients.
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Índice:
WPRIM
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Tropical Biomedicine
Año:
2022
Tipo del documento:
Article