Determination of azelaic acid and potassium azeloycinate diglycinate in cosmetics by HPLC / 上海预防医学
Shanghai Journal of Preventive Medicine
; (12): 1250-1256, 2022.
Article
en Zh
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| ID: wpr-964224
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ABSTRACT
ObjectiveAn HPLC method was established for the determination of azelaic acid and potassium azeloycinate diglycinate in cosmetics. MethodsThe samples were extracted with 60 mmol·L-1 sodium hydroxide water solution-methyl alcohol. After centrifugation and filtration, the analysis of azelaic acid and potassium azeloycinate diglycinate was performed with a SVEA C8(250 mm×4.6 mm, 5 μm) column, using 15 mmol‧L-1 potassium dihydrogen phosphate solution (pH=3.0) and acetonitrile for gradient elution at a flow rate of 1.0 mL·min-1.The analytes were detected with UV detector, and quantified by external standard curve. ResultsThe results showed a good linearity in the range of 5‒1 000 μg‧mL-1 with correlation coefficients (r) larger than 0.999. The detection limit of azelaic acid and potassium azeloycinate diglycinate (LOD) was 0.020% and 0.015%, respectively. The spiked recoveries were 87.66% to 108.96% with the relative standard deviation (RSD) of 0.6% to 3.3%. ConclusionThe method is simple, rapid and highly sensitive. It is suitable for the determination of azelaic acid and potassium azeloycinate diglycinate in cosmetics.
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Shanghai Journal of Preventive Medicine
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2022
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Article