Transcriptome analysis of Cinnamomum longepaniculatum by high-throughput sequencing
Electron. j. biotechnol
; Electron. j. biotechnol;28: 58-66, July. 2017. tab, graf, ilus
Article
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| LILACS
| ID: biblio-1015852
Bibliothèque responsable:
CL1.1
ABSTRACT
Background:
Cinnamomum longepaniculatum is an important commercial crop and the main source of volatile terpenoids. The biosynthesis of key bioactive metabolites of C. longepaniculatum is not well understood because of the lack of available genomic and transcriptomic information. To address this issue, we performed transcriptome sequencing of C. longepaniculatum leaves to identify factors involved in terpenoid metabolite biosynthesis.Results:
Transcriptome sequencing of C. longepaniculatum leaves generated over 56 million raw reads. The transcriptome was assembled using the Trinity software and yielded 82,061 unigenes with an average length of 879.43 bp and N50 value of 1387 bp. Furthermore, Benchmarking Universal Single-Copy Orthologs analysis indicated that our assembly is 91% complete. The unigenes were used to query the nonredundant database depending on sequence similarity; 42,809 unigenes were homologous to known genes in different species, with an annotation rate of 42.87%. The transcript abundance and Gene Ontology analyses revealed that numerous unigenes were associated with metabolism, while others were annotated in functional categories including transcription, signal transduction, and secondary metabolism. The Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that 19,260 unigenes were involved in 385 metabolic pathways, with 233 unigenes found to be involved in terpenoid metabolism. Moreover, 23,463 simple sequence repeats were identified using the microsatellite identification tool.Conclusion:
This is the first detailed transcriptome analysis of C. longepaniculatum. The findings provide insights into the molecular basis of terpenoid biosynthesis and a reference for future studies on the genetics and breeding of C. longepaniculatum.Mots clés
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Terpènes
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Cinnamomum
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Séquençage nucléotidique à haut débit
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Transcriptome
Type d'étude:
Prognostic_studies
langue:
En
Texte intégral:
Electron. j. biotechnol
Thème du journal:
BIOTECNOLOGIA
Année:
2017
Type:
Article
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Project document